N-Methyl-3-(1-methyl-1H-pyrazo

An Isotope Coding Strategy for Proteomics Involving Both Amine and Carboxyl Group Labeling

A stable isotope coding strategy is described for the analysis of all types of tryptic peptides, including those that are N-terminally blocked and from the C-terminus of proteins. The method exploits differential derivatization

Blocking cDNA arrays printed on amine slides

so they can hybridize to the fluorescently labeled probe DNA . During the blocking step the array will disappear so be sure that the boundary of the array has been marked with a diamond pin as described in microarray construction. During the process, the exposed amine

Synthesis of (2S, 4S, 5S)-5-(t-Butoxycarbonylamino)-4-(t-Butyldimethylsilyloxy)-2-Isopropyl-7-Methyl Octanoic Acid

to replace combinattons of leucme coupled with valine in pepttde sequences. The preparation described is the final method developed, after many early less successful attempts. The synthesis mvolves (Fig. 1 ) the coupling of 1-bromo-5-methyl-2-hexene 3 and N