Boronic acid,B-[4-[(methylamin

Immunohistochemical Detection of Protein Oxidation

, almost all oxidative modifications of proteins are resulting in an increase of carbonylated proteins. The three major pathways leading to carbonyl group formation (protein oxidation) are shown in Fig. 1 . Carbonyl groups are introduced into proteins as a result

Assay for ß-galactosidase in E. Coli

, isopropyl-ß-thiogalactoside (IPTG), phenyl-ß-galactoside (PBG) and glucose (GLU).   We will use a non-biological (artificial) substrate for the enzyme, Ortho-nitrophenyl-ß-galactoside (ONPG). In the presence of ß-galactosidase ONPG is converted

DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES

should be processed as soon after it is collected as possible. Assign an accession number to specimen according to procedure in Laboratory Procedures Manual. B. Aseptic technique must be used when setting up cultures, preferably under a laminar flow hood