7-Bromo-2-chlorobenzo[d]oxazol

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer

In Situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional -Galactosidase Staining

than 5-bromo-4-chloro-3-indolyl-β-d -galactoside (X-gal). S-gal is advantageous where β-gal activity is limited including early stage mouse embryos. As a result of the increased sensitivity as well as the color compatibility of S-gal, we successfully combined

Detection of Cell Proliferation and Cell Fate in Adult CNS Using BrdU Double-Label Immunohistochemistry

of the thymidine analog 5-bromo-2′-deoxyuridine (BrdU), which gets incorporated into DNA during the S-phase, has been used to detect proliferating cells in many tissues including brain (1 ). Single-stranded DNA with incorporated BrdU