- 询价
- ITI Biochem
- 美国
- 12007-40-8
- 2025年07月19日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Dipotassium tetraborate;Potassium borate
- 库存：
98
- 供应商：
北京孚博
- CAS号：
12007-40-8
- 规格：
1g
CAS：12007-40-8
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文献和实验Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
for 1.5 hours in the 42℃ incubator/shaker.Remove from incubation. Add 1040 µL of 2M potassium chloride (for a final concentration of 160 mM; this step will precipitate proteins from the extract). Gently vortex to mix. Incubate on ice for 1 hour.Centrifuge
提取棉花RNA--RNAEXTRACTION METHOD FOR COTTON
7.5) and clarify by centrifugation for 10 min. 10.Add Potassium Acetate (KAc, pH 5.5) to 200 mM in RNA suspension and incubate for 15 min on ice. 11.Remove salt-insoluble material by centrifugation. 12.Precipitate RNA overnight by adding 2.5 volumes cold 100
提取棉花RNA--RNA EXTRACTION METHOD FOR COTTON
.8.Centrifuge for 20 min at 12,000g to collect RNA pellet. Wash pellet 2-3 times with 5 ml cold 2 M LiCl until supernatant is relatively colorless.9.Resuspend RNA pellet in 2 ml 10 mM Tris-HCl (pH 7.5) and clarify by centrifugation for 10 min.10.Add Potassium
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