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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Ethyl 6-aminoimidazo[1,2-a]pyridine-3-carboxylate 95+%
- 库存:
98
- 供应商:
北京孚博
- CAS号:
1196153-46-4
- 规格:
1g
CAS:1196153-46-4
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文献和实验30-50 μm fixed frozen/vibratome sections, mounted on slides Beakers to mix and hold solutions Incubation chamber Solutions Cresyl fast violet Glacial acetic acid 100% ethyl alcohol Chloroform 95% ethyl alcohol / 5% deionized H2
tube mix: 15 µl dH2O (times number of samples + 1) 70 µl 1MTris Ph 8.0 (times number of samples + 1) 20 µl 4 mM Acetyl CoA (times number of samples + 1) and 5 µl 14 C CAM (times number of samples). 13. Add 0.5 ml Ethyl Acetate to each tube. 14
in 30 µl Ethyl Acetate. 19. Spot on silica gel TLC (in 5 µl amounts). 20. Develop TLC in 200 ml Chloroform/Methyl-OH (95:5) until solution is almost at the top (app. 1 hr.). 21. Dry & expose against film ON.
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