4-Bromo-1-t-butyl-3-methylpyra

Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies

is used to protect the α-amino group of each residue. Those residues that have potentially reactive side chains are protected with acid-labile groups such as t-butyl. After removal of the Fmoc group with piperidine, the next protected amino

High Sensitivity Measurement of Estrone and Estradiol in Serum and Plasma Using LC-MS/MS

two-dimensional chromatographic separation and tandem mass spectrometry detection. Aliquots of serum or plasma samples are combined with stable isotope-labeled internal standard and estrogens are extracted with methyl t-butyl ether. The solvent is evaporated

Methods of Cellular Senescence Induction Using Oxidative Stress

senescence in vitro. Various approaches to evoke oxidative stresses, such as the exposures of cells to ultraviolet light, ethanol, tert-butyl hydroperoxide (t-BHP), and peroxide hydrogen (H2 O2 ), have been used to study the onset of cellular senescence