- 询价
- ITI Biochem
- 美国
- 117786-94-4
- 2025年07月19日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
O-(2-Carboxyethyl)polyethylene glycol 3,000
- 库存：
98
- 供应商：
北京孚博
- CAS号：
117786-94-4
- 规格：
1g
CAS：117786-94-4
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文献和实验by the addition of polyethylene glycol and collected by centrifugation. The DNA pellet is resuspended in a buffer containing cesium chloride and ethidium bromide, which is loaded into polyallomer tubes and subjected to ultracentrifugation overnight. The ethidium
should be between 8.4 - 8.9. Note : Lithium Acetate can be sterilized in the autoclave for 20 mins liquid cycle. Polyethylene glycol (PEG 50% w/v) The polyethylene glycol (PEG), MW 3350 (Sigma P3640) is made up to 50% (w/v) with dd water and filter
Agrobacterium-Mediated Transformation to Create an Insertion Library in Magnaporthe grisea
. Two transformation approaches were used to build a library of insertion strains in M. grisea . Polyethylene glycol/CaCl2 -mediated protoplast transformation was the initial method we used and resulted in the generation of just more than 17,000 insertion strain lines
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