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- 文献和实验
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- 保存条件:
详询
- 保质期:
详询
- 英文名:
ZIRCONIUM IV NEOALKANOLATO, TRIS(DIISOOCTYL-PHOSPHATO-O))
- 库存:
98
- 供应商:
北京孚博
- CAS号:
117101-65-2
- 规格:
1g
CAS:117101-65-2
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文献和实验Transition Metal Methods for Immobilization of Enzymes and Cells
The use of transition metal chemistry for the immobilization of biological molecules is long established and well documented (1 –17 ). This method is based on the chelation properties of transition metals, namely titanium(IV) and zirconium
Replication timing by density transfer
the pellets at -20o C. 7. Extract DNA ( smash-and-grab with glass beads), digest with the enzyme of choice in 0.1 ml of reaction mix per sample. [I resuspend the nucleic acids pellet in 0.05 ml of 10 mM Tris, 0.1 mM EDTA, and add an equal
RNase-free DNaseI (10 U/µl), 5 µl 0.1 M Tris-Cl pH 8.3, 5 µl 0.5 M KCl, 5 µl 15 mM MgCl 2 phenol extract once precipitate with 5 µl NaAcetate and 200 µl 100 % Ethanol incubate at least for 30 min at -80 °C spin, wash
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