TRANS-3-BROMO-β-NITROSTYRENE

In Situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional -Galactosidase Staining

than 5-bromo-4-chloro-3-indolyl-β-d -galactoside (X-gal). S-gal is advantageous where β-gal activity is limited including early stage mouse embryos. As a result of the increased sensitivity as well as the color compatibility of S-gal, we successfully combined

Immunohistochemical Detection of -Galactosidase or Green Fluorescent Protein on Tissue Sections

of reporter genes in particular cell types. The two most commonly used reporter genes are LacZ , which encodes the enzyme β-galactosidase (β-gal), and green fluorescent protein (GFP). β-Gal expression can be detected enzymatically, using 5-bromo-4-chloro

Rapid Identification of Cloned HIV-1 Fragments

-fragment of β-galactosidase were developed (pUC vectors) (3 ,4 ). Upon induction by IPTG (isopropyl-β-D -thio-galactopyranoside), the expressed β-galactosidase could cleave X-gal (5-bromo-4-choloro-3-indoyl-β-D -galactopyranoside) and turn the colonies