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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
1,1'-[1,4-Phenylenebis-(methylene)]bis-(4,4'-bipyridinium)bis-(hexafluorophosphate)
- 库存:
98
- 供应商:
北京孚博
- CAS号:
108861-20-7
- 规格:
1g
CAS:108861-20-7
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文献和实验) Older (pre-Long Ranger Gels) Method 10X EB 164.0 g Tris-OH 27.5 g Boric Acid 7.45 g disodium EDTA water to 1 l Acrylamide stock 38% Acrylamide 2% bis-acrylamide Gels (volume is for standard size sequencing gels) 8% gel: 40.4 g urea
Single Strand Conformation Polymorphism (SSCP) Analysis using Nondenaturing PAGE
. In nondenaturing PAGE the components used to synthesize matrix were acrylamide monomers, N, N-methylene bisacrylamide (Bis), ammonium persulphate (APS) and N,N,N’,N’-tetramethylenediamine (TEMED).Ammonium persulphate (APS) when dissolved in water generates free
Stock Solutions 1) 1.5M TrisHCl pH 8.9 - Keep RT. 2) 30% Acrylamide 0.8% Methylene bis Acrylamide. Keep 4°C. 3) 0.5M TrisHCl pH 6.8 - Keep RT. 4) 10% Ammonium Persulfate (APS). Keep 4°C less than 1 month. Running Buffer x1
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