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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Acetamide,N-(4-nitrophenyl)-
- 库存:
98
- 供应商:
北京孚博
- CAS号:
104-04-1
- 规格:
1g
CAS:104-04-1
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文献和实验to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
ELISA PROTOCOL 【Yale University 】
-nitrophenyl phosphate (5 mg/tablet). SigmaCat.# N93895) AP-conjugated secondary Ab: AP-Rabbit anti-mouse IgG(Zymed). Reconstitute in ddH2O as prescribed by manufacturer andaliquot and store at -70 °C.6) 100 mM EDTA pH
High-Throughput Screening Method for Lipases/Esterases
High-throughput screening (HTS) methods for lipases and esterases are generally performed by using synthetic chromogenic substrates (e.g., p -nitrophenyl, resorufin, and umbelliferyl esters) which may be misleading
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