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- 文献和实验
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- 保存条件:
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- 英文名:
E-2-Chloro-5,8-dimethyl-3-(2-nitro)vinylquinoline
- 库存:
98
- 供应商:
北京孚博
- CAS号:
1031929-39-1
- 规格:
1g
CAS:1031929-39-1
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
immediately before using. Stocks X-Phos (100X):10mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (also referred to as BCIP) in H20. Store in the dark as aliquots at -200 C. Can be frozen and thawed several times. NBT (50X):50 mg/ml nitro
M Tris pH 9.6 200 µl 1M MgCl2 500 µl 5 mg/ml BCIP in dimethyl formamide (BCIP= 5-bromo-4- chloro-3-indoyl phosphate, toluidine salt), US Biochemical). Store this stock at -20° wrapped in foil; it's light sensitive. 2.5 ml 2 mg/ml NBT (p-nitro blue
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
(Boehringer Mannheim Cat# 242 608)- Levamisole (Sigma, Cat# L-9756) - NaN3 (Sigma Cat# S-2002)- NBT: 4-Nitro blue tetrazolium chloride. (Boehringer Mannheim Cat# 1383 213)- p-Phenylenediamine (Sigma Cat# P-6001)- 0.1% Poly-L-lysine solution
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