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2-8°C
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文献和实验Purification of Recombinant Calpastatin Expressed in Escherichia coli
Calpastatin is an endogenous inhibitor protein specific for calpain (1 ,2 ). Other calpain-inhibiting reagents such as EDTA, E-64, leupeptin, and calpain inhibitor I (N -acetyl-L-leucyl-L-leucyl-L-norleucinal), are frequently used
Production and Crystallization of Recombinant JAK Proteins
drug discovery programs has provided a powerful rationale for chemical modification by allowing a unique glimpse of a bound inhibitor to its target. Such structural information has not only led to an improved understanding of the key drivers of potency
Experimental Applications: Human Acetylcholinesterase as a Model Nervous System Protein
A DNA sequence encoding the brain and muscle form of human AChE (AChE-T) was constructed in our laboratory from cloned cDNA and genomic sequences, and tentatively identified by its homology to known ChEs (Soreq et al., 1990). This putative
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