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- 2025年07月18日
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文献和实验Expression and Preparation of Fusion Proteins from Recombinant gt11 Phages
The phage λgt11 system has become increasingly popular for expression of cDNAs or genomic DNAs either in phage plaques or in bacteria lysogenized with recombinant phages (1 ,2 ). It offers the advantages of high cloning efficiency, high-level
Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
The cellular retinaldehyde-binding protein (CRALBP) is expressed at high levels in vertebrate visual trssue, where it may serve to modulate the interaction of 11-cis -retinol with visual-cycle enzymes in the retinal pigment epithelium (RPE
Preparation and Ligand Screening of a gt11 Lysogen Library
The λgt11 system has been designed to allow expression of cDNAs either in phage plaques or in bacteria lysogenized with recombinant λgtll phages (1 , 2 ). cDNAs are cloned into a unique EcoRl restriction site near the 3′ end of a truncated
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