IT2670 衣霉素 内质网应激 索莱宝

Tunicamycin是一种抗生素, 可抑制 N-糖基化并阻断 GlcNAc 磷酸转移酶 (GPT)。Tunicamycin 引起细胞内质网 (ER) 中未折叠蛋白的积累并诱导 ER 应激，并导致 DNA 合成受阻和 G1 期细胞周期停滞。

衣霉素是一种同源核苷抗生素的混合物, 为四种异构体A, B, C, D的混合物：
异构体A, n=8, C₃₇H₆₀N₄O₁₆, 816.90
异构体B, n=9, C₃₈H₆₂N4O₁₆, 830.93
异构体C, n=10, C₃₉H₆₄N₄O₁₆, 844.95
异构体D, n=11, C₄₀H₆₆N₄O₁₆, 858.99

使用本产品的案例（仅供参考）

In Vitro

Cell（Buffalo Ovarian Granulosa Cells; 1~5 μg/mL tunicamycin; 37?C, 5% CO2, 4–48 h）

Briefly, the culture medium was removed and replaced with the culture medium consisting of diluted tunicamycin (TM) (Solarbio, Beijing, China).  GCs were then subjected to increasing doses of TM at concentrations of 1, 1.5, 2, 2.5, and 5 μg/mL and cultured at 37?C for approximately 24–48 h in a 5% CO2 incubator.

来源文献：Yang W, Liu R, Sun Q, Huang X, Zhang J, Huang L, Zhang P, Zhang M, Fu Q. Quercetin Alleviates Endoplasmic Reticulum Stress-Induced Apoptosis in Buffalo Ovarian Granulosa Cells.   Animals (Basel).   2022 Mar 20;12(6):787.   doi: 10.3390/ani12060787.   PMID: 35327186;   PMCID: PMC8944572.

Cell（ human NSCLC cell lines A549, PC9, and H1299；0.5-2 μM Tunicamycin/TM；24-72h）

0.5, 1, and 2 μM TM were used for 48 h or 2 μM TM was used for 24, 48, and 72 h to induce ER stress.  Ten micromolar MG132 was used for 6 h prior to harvesting the cells, and 50 μg·mL?1 CHX was used for indicated time to detect protein degradation.  Ten micromolar Radezolid was used to inhibit RRBP1 expression.

来源文献：Wang W, Wang M, Xiao Y, Wang Y, Ma L, Guo L, Wu X, Lin X, Zhang P. USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer.  Mol Oncol. 2022 Apr;16(7):1572-1590.  doi: 10.1002/1878-0261.13112.  Epub 2021 Oct 18.  PMID: 34618999;  PMCID: PMC8978513.

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标题:USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer

成员:Wang W; Wang M; Xiao Y; Wang Y; Ma L; Guo L; Wu X;

论文因子:6.6 发表期刊:Mol Oncol pmid:34618999

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:Quercetin Alleviates Endoplasmic Reticulum Stress-Induced Apoptosis in Buffalo Ovarian Granulosa Cells

成员:Yang W; Liu R; Sun Q; Huang X; Zhang J; Huang L; Z

论文因子:3 发表期刊:Animals (Basel) pmid:35327186

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:Quercetin Alleviates Endoplasmic Reticulum Stress-Induced Apoptosis in Buffalo Ovarian Granulosa Cells

成员:Yang W; Liu R; Sun Q; Huang X; Zhang J; Huang L; Z

论文因子:3 发表期刊:Animals (Basel) pmid:35327186

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:Low-intensity pulsed ultrasound upregulates osteogenesis under inflammatory conditions in periodontal ligament stem cells through unfolded protein response

成员:Li H; Deng Y; Tan M; Feng G; Kuang Y; Li J; Song J

论文因子:5.081 发表期刊:Stem Cell Res Ther pmid:32493507

基本信息
CAS	No.11089-65-9
中文名称	衣霉素
英文名称	Tunicamycin
分子式	Mixture
溶解性	Soluble in DMSO
纯度	≥98%
外观(性状)	White to light yellow Solid
储存条件	Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
EC	EINECS 601-012-4
MDL	MFCD00065709
SMILES	O=C1NC(N([C@H]2[C@H](O)[C@H](O)[C@@H](C(C[C@@H]3[C@H](O)[C@H](O)[C@@H](NC(/C=C/[(CH2) 8-11]C(C)C)=O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4NC(C)=O)O3)O)O2)C=C1)=O
靶点	Bacterial；Fungal；Influenza Virus；Antibiotic；ER Stress
通路	Anti-infection；ER Stress
背景说明	Tunicamycin是一种同源核苷抗生素的混合物, 可抑制 N-糖基化并阻断 GlcNAc 磷酸转移酶 (GPT)。Tunicamycin 引起细胞内质网 (ER) 中未折叠蛋白的积累并诱导 ER 应激，并导致 DNA 合成受阻和 G1 期细胞周期停滞。
单位	瓶