- ￥440
- Solarbio
- 北京
- IE05001
- 2025年07月23日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
Ethyl gallate(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
831-61-8
- 规格：
1ml
| 基本信息 | |
| CAS | No.831-61-8 |
| 中文名称 | 没食子酸乙酯(10mM in DMSO,无菌） |
| 英文名称 | Ethyl gallate(10mM in DMSO,Sterile） |
| 分子式 | C9H10O5 |
| 分子量 | 198.17 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | Others |
| 通路 | Others |
| 背景说明 | 是一种非类黄酮酚化合物和过氧化氢的清除剂。 |
| 生物活性 | Ethyl gallate is a nonflavonoid phenolic compound and also a scavenger of hydrogen peroxide.[1-2] |
| In Vitro | Ethyl gallate is a nonflavonoid phenolic compound and also a scavenger of hydrogen peroxide. After treatment for 24 h or 48 h with Ethyl gallate， HL-60 cells show changes in morphology， including shrinkage of the cell membrane and the development of apoptotic bodies. Consistent with these effects， the viability of Ethyl gallate-treated cells decreases in a time- and dose-dependent manner， demonstrating that Ethyl gallate has a cytotoxic effect on HL-60 cells. Ethyl gallate treatment increases the proportion of cells in subG1 phase in a concentration- and time-dependent manner. Treatment of cells for 24 h or 48 h with 50 μM or 75 μM Ethyl gallate increases the percentage of cells in the subG1 phase from a baseline of 2.9% to 26.5% or 52.6%， respectively. It is found that Ethyl gallate treatment of HL-60 cells decreases the expression of Bcl-2 at 75 μM Ethyl gallate， and increases Bax and truncated Bid (tBid) expression at 24 h[1]. |
| 细胞实验 | No significant difference in the serum total protein， albumin， globulin and glucose is found between the rats fed with A. nilotica (L.) leaf extract on ethyl gallate equivalent basis and those fed with Ethyl gallate alone. Significant differences in total bilirubin level， however， exist between the rats that receive A. nilotica (L.) leaf extract， 500 mg/kg body weight (ethyl gallate equivalent of 10 mg/kg， 0.34±0.01 mg/dL) and those receiving 10 mg/kg body weight of Ethyl gallate (0.26±0.01 mg/dL). Significant difference is found for ALT between groups fed with 500 and 1000 mg/kg body weight of A. nilotica (L.) leaf extract (26.52±1.23 and 30.05±1.38 U/L) and 10 and 20 mg/kg of Ethyl gallate (20.50±0.94 and 24.67±1.13 U/L)[2]. |
| 细胞实验 | HL-60 cells (1×106) are treated with 50 μM or 75 μM Ethyl gallate for 24 h or 48 h at 37°C. Cells are then harvested by centrifugation and fixed in 70% ethanol at 4°C for 24 h. Fixed cells are resuspended in PBS containing 40 μg/mL Propidium iodide (PI)， 100 μg/mL RNase A， and 0.1% Triton X-100 and incubated in the dark for 30 min at room temperature. Cell cycle distribution is analyzed by flow cytometry on a FACSCalibur. To investigate apoptotic cells， HL-60 cells (1×106) incubated with different concentration of 50 μM， 75 μM and 100 μM Ethyl gallate for 24 h or 48 h at 37°C， and then DAPI staining is conducted. The cells are photographed using a fluorescence microscopy[1]. |
| 动物实验 | Forty eight female albino Wistar rats of six to eight weeks old are used and divided into eight groups based on their body weights. Group 1 rats serve as control receiving 1.0 mL of the vehicle (0.1% ethanol); Group 2 rats receive A. nilotica (L.) leaf extract (250 mg/kg body weight); Group 3 rats receive A. nilotica (L.) leaf extract (500 mg/kg body weight); Group 4 rats receive A. nilotica (L.) leaf extract (1000 mg/kg body weight); Group 5 rats receive A. nilotica (L.) leaf extract (2000 mg/kg body weight); Group 6 rats receive Ethyl gallate (5 mg/kg body weight); Group 7 rats receive Ethyl gallate (10 mg/kg body weight); Group 8 rats receive Ethyl gallate (20 mg/kg body weight). Body weights are recorded on 0th and 14th day for each group and all rats are decapitated after an overnight fast[2]. |
| 激酶实验 | The expression of apoptosis-related proteins (caspases-8， -9， -3; AIF; Endo G; Bid; Bax; and Bcl-2) in HL-60 cells is determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of lysates followed by western blotting. For this， HL-60 cells (1.5×106) are treated with 50 μM or 75 μM Ethyl gallate for 6 h， 12 h， or 24 h. Total cell lysates are obtained by resuspending cells in ice-cold radioimmunoprecipitation assay (RIPA) buffer for 30 min followed by centrifugation. Protein concentration is determined using a NanoDrop spectrophotometer. Aliquots of lysates (100 μg protein equivalents) are resolved by 12% SDS-PAGE and transferred onto nitrocellulose membranes[1]. |
| 数据来源文献 | [1]. Kim WH， et al. Ethyl gallate induces apoptosis of HL-60 cells by promoting the expression of caspases-8， -9， -3， apoptosis-inducing factor and endonuclease G. Int J Mol Sci. 2012;13(9):11912-22. [2]. Mohan S， et al. In vitro protection of biological macromolecules against oxidative stress and in vivo toxicity evaluation of Acacia nilotica (L.) and ethyl gallate in rats. BMC Complement Altern Med. 2014 Jul 21;14:257. |
| 单位 | 瓶 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
IE05001 没食子酸乙酯(10mM in DMSO,无菌） 抑制剂/拮抗剂/激动剂 索莱宝
￥440
