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- 2025年07月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
1,5-Dicaffeoylquinic acid(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
19870-46-3
- 规格：
1ml
| 基本信息 | |
| CAS | No.19870-46-3 |
| 中文名称 | 1,5-二咖啡酰奎宁酸(10mM in DMSO,无菌） |
| 英文名称 | 1,5-Dicaffeoylquinic acid(10mM in DMSO,Sterile） |
| 分子式 | C25H24O12 |
| 分子量 | 516.45 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | Others |
| 通路 | Others |
| 背景说明 | 1，5-Dicaffeoylquinic acid是一种具有生物活性的化合物。具有抗氧化，抗胆碱能，还原能力，自由基清除和金属结合活性。 |
| 生物活性 | 1，3-Dicaffeoylquinic acid is a caffeoylquinic acid derivative that exhibits antioxidant activity and radical scavenging activity.[1-3] |
| In Vitro | 1，3-Dicaffeoylquinic acid shows increased neuronal cell viability against Aβ(42) toxicity in a concentration-dependent manner in neurons. 1，3-Dicaffeoylquinic acid activates both phosphoinositide 3-kinase (PI3K)/Akt and extracellular regulated protein kinase 1/2 (Erk1/2) with stimulating their upstream tyrosine kinase A (Trk A). 1，3-Dicaffeoylquinic acids anti-apoptotic potential is related to the enhanced inactivating phosphorylation of glycogen synthase kinase 3β (GSK3β) and the modulation of expression of apoptosis-related protein Bcl-2/Bax[2]. 1，3-Dicaffeoylquinic acid (10 μM， 20 μM， 50 μM， and 100 μM) significantly increases cell viablity before OGD/reperfusion， and prevents the depletion of GSH under OGD/reperfusion insult. 1，3-Dicaffeoylquinic acid induces nuclear translocation of Nrf2 in OGD/reperfusion treated astrocytes， and induces increased GCL activity， and the effect is lost in Nrf2 siRNA-transfected cells[3]. |
| 细胞实验 | 1，3-Dicaffeoylquinic acid (32.0 mg/kg， p.o.) and 1-O-ABL are absorbed very quickly in Wistar rats. The maximum plasma concentrations for 1，3-Dicaffeoylquinic acid and 1-O-ABL are 44.5 ± 7.1 and 19.1 ± 6.9 ng/mL， respectively[1]. |
| 细胞实验 | The viability of astrocytes is measured by the MTT reduction method. Briefly， the cells are rinsed with phosphate-buffered saline， pH 7.2， and incubated with 5 mg/mL MTT reagent for 3 h at 37°C. The medium is removed， and the cells are lysed with 1 mL of dimethyl sulfoxide. The absorbance is measured at 540 nm by a microplate reader[3]. |
| 动物实验 | Six male Wistar rats (200-250 g) are fasted for 12 h with free access to water prior to oral administration of I. britannica extract with an herb dose of 8.0 g/kg (equivalent to 32.0 mg/kg 1，3-Dicaffeoylquinic acid， and 4.01 mg/kg， 1-O-ABL). Blood samples (appr 0.25 mL) are collected from suborbital vein into heparinized tubes at 0， 0.08， 0.16， 0.33， 0.67， 1， 1.5， 2， 4， 6， 9 and 12 h after dosing， and then immediately centrifuged at 3500 rpm for 10 min. Harvested plasma samples are stored at -60°C until analysis. The plasma concentrations of 1，3-Dicaffeoylquinic acid and 1-O-ABL are calculated from the calibration curves obtained daily[1]. |
| 激酶实验 | The whole cellular lysate is prepared using a RIPA Lysis Buffer added to a reaction buffer containing 0.1 M Tris (pH 8.2)， 0.15 M KCl， 10 mM ATP， 10 mM l-glutamate， 20 mM MgCl2， and 2 mM EDTA at 37°C for 3 min， and then 5 mM cysteine is added at 37°C for 15 min. The production of glutamylcysteine is immediately quantified for HPLC analysis by O-phthalaldehyde derivatization. GCL activity is presented in units of femtomoles of -GC produced per milligram of protein per minute[3]. |
| 数据来源文献 | [1]. Wang Z， et al. An LC-MS/MS method for simultaneous determination of 1，5-dicaffeoylquinic acid and 1-O-acetylbritannilactone in rat plasma and its application to a pharmacokinetic study. [2]. Xiao HB， et al. 1，5-dicaffeoylquinic acid protects primary neurons from amyloid β 1-42-induced apoptosis via PI3K/Akt signaling pathway. Chin Med J (Engl). 2011 Sep;124(17):2628-35. [3]. Cao X， et al. 1， 5-Dicaffeoylquinic acid-mediated glutathione synthesis through activation of Nrf2 protects against OGD/reperfusion-induced oxidative stress in astrocytes. Brain Res. 2010 Aug 6;1347:142-8. |
| 单位 | 瓶 |
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ID09001 1,5-二咖啡酰奎宁酸(10mM in DMSO,无菌） 免疫学/炎症 索莱宝
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