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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
Ellagic acid(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
476-66-4
- 规格：
1ml
| 基本信息 | |
| CAS | No.476-66-4 |
| 中文名称 | 鞣花酸(10mM in DMSO,无菌） |
| 英文名称 | Ellagic acid(10mM in DMSO,Sterile） |
| 分子式 | C14H6O8 |
| 分子量 | 302.19 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | Casein Kinase（CK） |
| 通路 | Cell Cycle;DNA Damage/DNA Repair; Stem Cells |
| 背景说明 | Ellagic acid是一种抗氧化剂，为有效的，ATP 竞争性的 CK2 抑制剂。 |
| 生物活性 | Ellagic acid is a natural antioxidant， and acts as a potent and ATP-competitive inhibitor of CK2 and SHP2， with an IC50 of 40 nM and a Ki of 20 nM.[1-4] |
| In Vitro | Ellagic acid is a potent CK2 inhibitor， with an IC50 of 40 nM and a Ki of 20 nM. Ellagic acid also blocks other kinases such as LYN， PKA， SYK， GSK3， FGR and CK1， with IC50s of 2.9， 3.5， 4.3， 7.5， 9.4 and 13.0 μM， respectively， and shows no obvious effects on DYRK1a， CSK， NPM-ALK， RET and FLT3 (IC50s > 40 μM). Ellagic acid (5-100 μM) shows inhibitory activities against Karpas299， SUDHL1， SR786， and FE-PD cell lines[1]. Ellagic acid (10 μM) exhibits cytotoxic effects on MCF-7 cells after treatment of radiation. Ellagic acid (10 μM) in combination with Irradiation (IR) significantly abridges the capacity of MCF-7 cells to form colonies equated with individual treatments. Ellagic acid with IR also induces cell apoptosis， and facilitates the upregulation of pro-apopttotic Bax and downregulation of Bcl-2 in MCF-7 cells[3]. |
| 细胞实验 | Ellagic acid (EA; 10 mg/kg/day; p.o.， 14 days) strongly decreases MDA brain content by 17%， and reduces the levels of brain TNF-α by 42% in rats. Ellagic acid markedly increases the reduced brain contents of 5-HT (39%)， dopamine (DA， 71%)， and norepinephrine (NE， 77%). Ellagic acid (10 mg/kg， p.o.， 14 days) causes decreased histopathological changes induced by Doxorubicin in rats[2]. |
| 细胞实验 | ALCL cell viability is measured by MTT assay. Briefly， 0.1 × 105 cells are seeded onto 96-well microculture plates 12 hrs before adding ellagic acid. The cells are grown in 200 μL of complete RPMI-1640 medium， under standard tissue-culture conditions， in the presence or absence of the drug (Ellagic acid) for 48 hours. Twenty μL of MTT solution (5 mg/mL) are then added to the cell suspension for 4h. The intracellular formazan crystals are dissolved with 150 μL of DMSO and optical density， measured on a spectrophotometer at 540 nm， represents the mean (± SD) of triplicate cultures[1]. |
| 动物实验 | Fifty male adult Sprague-Dawley rats are divided randomly into five groups as follow: Group (1) receives corn oil orally as a vehicle and served as normal control. Group (2) receives doxorubicin (DOX) injection (5 mg/kg， i.p.) twice a week for 14 days. Group (3) receives Ellagic acid (10 mg/kg， p.o.; daily) for 14 days and DOX (5 mg/kg， i.p.) twice a week for 14 days. Group (4) receives rosmarinic acid (RA; 75 mg/kg， p.o.; daily) for 14 days and DOX (5 mg/kg， i.p.) twice a week for 14 days. Group (5) receives Ellagic acid (10 mg/kg， p.o.; daily) with RA (75 mg/kg， p.o.; daily) for 14 days and DOX injection (5 mg/kg， i.p.) twice a week for 14 days[2]. |
| 激酶实验 | CK2 and CK1 phosphorylation assays are carried out at 37°C in the presence of increasing amounts of each inhibitor (Ellagic acid) in a final volume of 25 μL containing 50 mM， Tris-HCl pH 7.5， 100 mM NaCl， 12 mM MgCl2， 0.02 mM [33P-ATP] (500-1000 cpm/pmol)， unless otherwise indicated. The phosphorylatable substrates are the synthetic peptide substrate RRRADDSDDDDD (100 μM) and RRKHAAIGDDDDAYSITA (200 μM) for CK2 and CK1， respectively. Reaction started with the addition of the kinase and is stopped after 10 min. by addition of 5 μL of 0.5 M orthophosphoric acid before spotting aliquots onto phosphocellulose filters. Filters are washed in 75 mM phosphoric acid substrate following SDS-PAGE of the radiolabeled samples. DYRK1A， assayed on the peptide RRRFRPASPLRGPPK， and tyrosine kinase activities are determined[1]. |
| 数据来源文献 | [1]. Cozza G， et al. Identification of ellagic acid as potent inhibitor of protein kinase CK2: a successful example of a virtual screening application. J Med Chem. 2006 Apr 20;49(8):2363-6. [2]. Rizk HA， et al. Prophylactic effects of ellagic acid and rosmarinic acid on doxorubicin-induced neurotoxicity in rats. J Biochem Mol Toxicol. 2017 Dec;31(12). [3]. Ahire V， et al. Ellagic Acid Enhances Apoptotic Sensitivity of Breast Cancer Cells to γ-Radiation. Nutr Cancer. 2017 Aug-Sep;69(6):904-910. [4]. Ma CH， et al. Discovery of ellagic acid as a competitive inhibitor of Src homology phosphotyrosyl phosphatase 2 (SHP2) for cancer treatment: In vitro and in silico study. Int J Biol Macromol. 2023 Nov 5;254(Pt 2):127845. |
| 单位 | 瓶 |
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。 一、适应症各种不明原因产生的腹水，肝硬化出现腹水、腹腔内注射药物和拟行腹水回输者。 二、标本采集①在无菌条件下有临床医师行穿刺采集。②送检标本最好留取中段液体于消毒容器内。常规及细菌学检查约留取2ml，生化检验留2ml，厌氧菌培养留1ml，如查结核杆菌则约需10ml。 3、为防止出现凝块、细胞变性、细菌破坏自溶等，除应及时送检外，常规及细菌学检查宜用1/60标本量的100g/L EDTA Na2抗凝，并立即离心浓集细胞，否则应在标本内加入乙醇至10%的浓度，置冰箱
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IE00501 鞣花酸(10mM in DMSO,无菌） 细胞周期 索莱宝
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