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- 禾午生物
- P74598
- 国产
- 2025年07月18日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pX333-U6-Atg16l1(rat)-U6-Atg16l2(rat)-sgRNA2-CBH-Cas9-Puro
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pX333-U6-Atg16l1(rat)-U6-Atg16l2(rat)-sgRNA2-CBH-Cas9-Puro |
| 别称 | gagtgtccacgacatcttgg；ttgctcgcgcagagtctcgt |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 基因编辑 |
| 片段类型 | CRISPR |
| 片段物种 | 大鼠 |
| 原核抗性 | Amp |
| 筛选标记 | Puro |
| 启动子 | U6 |
| 复制子 | pUC |
| 感受态 | Stbl3 |
| 温度 | 37度 |
| 背景载体 | pX333-2×(U6-gRNA)-CBH-Cas9-Puro |
| pCMV-3×FLAG-MBD2(human)-Neo |
| pCMV-Agtpbp1(mouse)-3×Myc-Neo |
| pCMV-APOE(human)-Neo |
| pCMV-UGP2(human)-Neo |
| pCMV-NLS-EGFR(human)-NLS-3×HA-Neo |
| pCMV-HERC4(human)-3×HA-Neo |
| pCMV-Hat1(mouse)-3×Myc-Neo |
| pCMV-EGFP-UMOD(human)-Neo |
| pCMV-EGFP-HEIH(human)-lncRNA-Neo |
| pCMV-EGFP-ATP2A2(human)-Neo |
| pCMV-Ttll7(mouse)-3×FLAG-Neo |
| pCMV-DACH2(human)-Neo |
| pCxcl10(mouse)-Fluc |
| pNos2(mouse)-Fluc |
| pCMV-Ppp1r14a(mouse)-3×FLAG-Neo |
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文献和实验There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
The first transgenic experiment was carried out more than 15 yr ago, and transgenesis is now a routine technique for the study of gene and cell function, and the development of animal models of disease. The mouse remains the species of choice
There is a pre-training phase and a memory probe phase. The pre-training phase last 1 week. The memory probe phase last 40 days or until criterion are met. Rat training should begin at a similar time every day. Training
技术资料