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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Cerium (IV) Sulfate
- 库存:
98
- 供应商:
北京孚博
- CAS号:
10294-42-5
- 规格:
1g
CAS:10294-42-5
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文献和实验Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
Isolation of Covalent Enzyme-DNA Complexes
. This covalent complex can be irreversibly trapped by treatment with strong protein denaturants, such as sodium dodecyl sulfate (SDS) or alkali. Type II topoisomerases (i.e., bacterial gyrase and topoisomerase IV, bacteriophage T4 topoisomerase II, African swine
Large scale nuclear extract preparation
pellets in nuclear lysis buffer (5ml/pellet).Perform one stroke with Dounce homogenizer 3 Add lysis buffer to 40ml and 2ml of 4M ammonium sulfate and agitate for 30-60min at 4℃ IV.Reprecipitate nuclear proteins 1.Centrifuge lysates in 55.2Ti rotor (35
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