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- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
DECAFLUORO-4-(PENTAFLUOROETHYL)CYCLOHEXANESULFONIC ACID POTASSIUM SALT
- 库存:
98
- 供应商:
北京孚博
- CAS号:
335-24-0
- 规格:
1g
CAS:335-24-0
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文献和实验常用化学试剂分子量 Molecular Weights of Commonly Used Chemicals
Ammonium Sulfate 132.14 Polyvinylpyrrolidone 40,000 Ampicillin 371.4 Potassium Acetate 98.14 ATP, disodium salt 605.19
DNA Purification by phenol extraction and ethanol precipitation
Equipment and reagents 1. Phenol 2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM EDTA,pH 8.0) 3. 24:1 (v/v) chloroform-isoamyl alcohol 4. 3 M potassium acetate, pH 5.5,prepared by adding glacial acetic acid to 3 M potassium acetate
DNAPurificationbyphenolextractionandethanolprecipitation
acid to 3 M potassium acetate until this pH is obtained 5. Microcentrifuge Method 1. Add an equal volume of phenol to the DNA containing reaction mixture and vortex gently. 2. Separate the aqueous phase which contains the DNA from the organic
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