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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
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详询
- 英文名:
Cyclopenta[b]pyrrol-2(1H)-one, hexahydro-, (3aR-cis)- (9CI)
- 库存:
98
- 供应商:
北京孚博
- CAS号:
131348-75-9
- 规格:
1g
CAS:131348-75-9
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文献和实验min. 3. Inhibit translation by adding to the medium a cycloheximide/chloramphenicol mixture in a final concentration of 100 μg/ml and 40 μg/ml (no-translation mix, NTM), respectively. 4. Incubated cells with translation inhibitors for 1h
Metabolic labeling Immunoprecipitation
once and replace with fresh medium (3ml/well).Metabolic labeling 1.Transfected cells -> 24h after medium change,rinse cells twice with Labeling Medium. 2.Add 1.5ml/well Labeling Medium 3.Add 20-30µl (~250µCi)of [35 S] methionine/cysteine /well 4.Culture 1h
Metabolic labeling Immunoprecipitation
cells twice with Labeling Medium. 2. Add 1.5ml/well Labeling Medium 3. Add 20-30µl (~250µCi) of [35 S] methionine/cysteine /well 4. Culture 1h at 37°C (pulse) 5. Add 1.5ml/well Chase Medium 6. Culture o/n (chase) 7. Harvest metabolically
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