α-Chloro-3-nitrobenzaldoxime

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

an all-in-one universal vector (pSC101BADγβαA-I-SceI [amp]) to be used in E. coli. This system expresses the recombinases Redγ, Redβ and Redα as well as the I-SceI homing endonuclease under the tight control of L -arabinose and chloro-tetracycline, respectively, and makes

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

Synthesis of Nucleoside 5′‐(α‐P‐Thio)Triphosphates Basic Protocol 3: One‐Pot Synthesis of Nucleoside 5′‐(α‐P‐Seleno)Triphosphates Commentary Literature Cited Figures

分子实验方法5:重组质粒的连接、转化及筛选

DNA的重组质粒和无插入而自身环化的载体分子是较为困难的。通过调整连接反应中外源DNA片段和载体DNA的浓度比例,可以将载体的自身环化限制在一定程度之下,也可以进一步采取一些特殊的克隆策略,如载体去磷酸化等来最大限度的降低载体的自身环化,还可以利用遗传学手段如α互补现象等来鉴别重组子和非重组子。 　　外源DNA片段和质粒载体的连接反应策略有以下几种： 　　1、带有非互补突出端的片段 用两种不同的限制性内切酶进行消化可以产生带有非互补的粘性末端,这也是最容易克隆的DNA