- 询价
- ITI Biochem
- 美国
- 75535-31-8
- 2025年07月18日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
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详询
- 英文名：
Calcium chloride fluoride phosphate
- 库存：
98
- 供应商：
北京孚博
- CAS号：
75535-31-8
- 规格：
1g
CAS：75535-31-8
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文献和实验CALCIUM CHLORIDE TRANSFORMATION OF BACTERIA WITH PLASMIDS
Preparation of Transformation Competent DNA Dilute an overnight bacterial culture 1:20 in L broth. (For E. coli strain JA221, at A ) Using a serological pipet, resuspend the bacterial pellet gently in 1/2 the culture volume of cold 50 mM CaCl
Simplified Calcium Phosphate Coprecipitation
Materials 2x HEPES: 8 g NaCl, 0.105g NaHPO4 , 6.5 g HEPES, H2O to 500 mL, pH 6.95 to 7.10 (try a range) 2M CaCl2 : store in aliquots at -20°C DNA 4 mg/mL Procedure (The volumes can be scaled
DNA Transfection: Calcium Phosphate Method
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA–calcium phosphate precipitates are internalized
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