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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
7,4'-Di-O-methylapigenin 5-O-xylosylglucoside
- 库存:
98
- 供应商:
北京孚博
- CAS号:
221257-06-3
- 规格:
1g
CAS:221257-06-3
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文献和实验The glucosylation of 5-position of anthocyanin molecules is one of most important modification step, which is responsible for the stability and color hue of anthocyanin molecules. However, anthocyanin 5-O -glucosyltransferase (5-GT) is unstable
for epithelial cells 2 x 105 cells/ml for fibroblast cells 5. Add 100 m l/cell to 96 well culture plates. 6. Incubate overnight at 37o C. 7. Wash plates twice with PBS 8. Add 125 m l/well 10% Buffered Formalin 9. Fix for 15
室一般用6.5%的非变性胶.制胶很重要,直接影响电泳的效果!一般控制在5分钟凝固的效果. 10X TBE 1.0ml 40% Acrylamide(40%聚丙烯酰胺) 3.3ml 50% Glycerol(50%甘油) 1.0ml dH2O(蒸馏水) 14.8ml TEMED(四甲基乙二氨) 20μl 脱气10min 10% AP(过硫酸氨) 120μl 总量 20.0ml 2. 一般试剂盒包括的试剂 10X Binding
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