6,7-Dihydro-5H-cyclopenta[b]py

IF=20.3！外泌体热点之牙周组织再生研究

活性和矿化结节形成，具有 PDLCs 亲和力和骨诱导潜能。接着作者利用 miRNA 测序和生物信息学分析，作者确定了前 20 个最丰富的 miRNAs，其中 miR-148a-3p、miR-423-5p、miR-100-5p 和 miR-125b-5p 的表达水平高于亲本 DFSCs，ARS 和 ALP 染色结果显示，miR-423-5p 在 DFSC-sEVs 中表达水平最高，且有最显著的增强成骨分化效果。这些结果表明 miR-423-5p 是 DFSC-sEVs 中促进 PDLCs 成骨

Preparation of Unnatural Amino Acids with Ammonia-Lyases and 2,3-Aminomutases

of unnatural amino acids. Besides the synthesis of various unnatural α-amino acids with the aid of phenylalanine ammonia-lyases (PALs) utilizing the 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) prosthetic groups, the biotransformations leading to various

Preparation of probes for in situ hybridization

. Labeling is then conducted for 24-48 h in the dark, at room temperature, with occasional vortexing. NB: Any trace of free amine, such as Tris base, should be removed from the nucleic acid, as it will also react with the fluorophore.