- 询价
- ITI Biochem
- 美国
- 98594-72-0
- 2025年07月18日
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![[(2R,3R,4R,5R)-5-(4-amino-5-hexyl-2-oxo-pyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxyphosphonic acid](https://img1.dxycdn.com/p/s14/2025/0214/009/6125451069768820981.jpg!wh200)
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
5-Pyrimidinecarboxylic acid, 1,4-dihydro-2-[(1-methylethyl)amino]-4-oxo- (9CI)
- 库存：
98
- 供应商：
北京孚博
- CAS号：
98594-72-0
- 规格：
1g
CAS：98594-72-0
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文献和实验with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 5 X 105 cells/ml. 4) Add [3H] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA
] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA concentration is in the nM range. 5) Incubate cells with label for a period of 60 minutes (2 hours???). 6) Remove label by washing cells 3 X with ice cold media
Arachidonic Acid Labeling 花生死烯酸标记方法
serum free media and supplement with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin).--> "Appropriate" = bring cells to a final concentration of 5 X 105 cells/ml.4) Add [3H] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???).--> Make
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