5-FLUORO-2'-O-METHYL-4-THIOURI

Treating Respiratory Viral Diseases with Chemically Modified, Second Generation Intranasal siRNAs

) that were chemically modified and screened for improved stability, activity and pharmacokinetics. 2′-O -methyl (2′-O -Me) and 2′-deoxy-2′-fluoro (2′-F) substitutions in the ribose ring were incorporated in different positions of the sense and antisense strands

Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection

-4-sulfonate (15 ,16 ), methyl 4-(6-methoxynaphthalene-2-yl)-4-oxo-2-butenoate (17 ), N-(1-pyrenyl)maleimide (18 ), monobromobimane (19 –23 ), o -phthaldialdehyde (24 ,25 ), 9-fluorenylmethyloxycarbonyl chloride (26 ), and 2-chloro-1-methylpyridinium

DNA甲基化研究方法的回顾与评价（下）

probe amplification，MS-MLPA) Anders O.H.等2005年改进MLPA[44]为MS-MLPA[45]用于检测特异位点的甲基化。MS-MLPA中，针对甲基化的和非甲基化的位点分别设计两个探针，每个探针都包括两个寡核苷酸部分，其中短的部分由合成产生，长的部分来源于phageM13的衍生物，后者有一个非杂交填充片段，不同探针其长度不同。探针的两个寡核苷酸杂交序列均与目标序列互补，其末端都连有相同的PCR引物。且要求[45]设计的MS-MLPA的探针要有甲基化敏感的限制性内切