- 询价
- ITI Biochem
- 美国
- 618091-96-6
- 2025年07月18日
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5-[(3S)-Dithiolan-3-yl]pentanoic acid
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- 文献和实验
- 技术资料
- 英文名：
(5-AMINO-1-(4-BROMOPHENYL)-1H-PYRAZOL-4-YL)(2-BROMOPHENYL)METHANONE
- 库存：
98
- 供应商：
北京孚博
- CAS号：
618091-96-6
- 规格：
1g
CAS：618091-96-6
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文献和实验deionized formamide 500 m l 10X TBE 500 m l 0.2% bromophenyl blue and 0.2% xylene cyanol Other Reagents Needed: 0.22 m M disposable syringe filters, short wave UV source, intensifying screen for UV shadowing Procedure • Pour a 20% denaturing gel: 12 ml
Protein Gel Preparation and Staining
250 mM Tris Base 1.65 g Tris Base 250 mM glycine 9.35 g glycine 0.1% SDS 2.5 ml 20% SDS up to 500 ml with Q 5X Loading Dye 0.5 M Tris 6.8 5 ml 1M Tris pH 6.8 50% glycerol 5 ml glycerol 0.5% bromophenyl blue 0.05 g
In vitro transcription with yeast nuclear extract
). Incubate 3 min at room temp. 6. Add 3 microliters sequencing gel loading buffer (formamide/EDTA plus bromophenyl blue and xylene cyanol) and mix well. 7. Heat 1 min at 90 - 100 degrees and immediately put on ice. 8. Load to 6% DNA sequencing gel and run
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