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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
4-THIOURIDINE 5'-DIPHOSPHATE AMMONIUM
- 库存:
98
- 供应商:
北京孚博
- CAS号:
108322-05-0
- 规格:
1g
CAS:108322-05-0
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文献和实验Ranger (50%) 8 ml 8 ml 9.6 ml water 30.5 ml(35.8 ml) 27.8 ml(34.2 ml) 28.9 ml (34.2 ml) ammonium
. Incubate the samples for 30 min at 37°C. 22. Extract once with an equal volume of Phenol:Chloroform. Mix well. 23. Centrifuge at 16,000 X g at room temperature for 10 min. 24. Collect the upper, aqueous phase. 25. Add 7.5 M Ammonium Acetate, pH
at room temperature for 10 min. 24. Collect the upper, aqueous phase. 25. Add 7.5 M Ammonium Acetate, pH 7.7 to a final concentration of 2.5 M and 2.5 volume of ice-cold 100% Ethanol to precipitate the DNA. 26. Centrifuge at 10,000 X g for 10
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