4-Hydroxy-3-methylphenyl thioc

RNA Extraction by the Guanidine Thiocyanate Procedure

This method relies on the strong chaotropic nature of the reagents involved to completely denature any ribonuclease (RNase) present in the sample. After lysis in guanidine thiocyanate buffers there are two possibilities for isolation

Acid-guanidinium thiocyanate-phenol-chloroform RNA purification

    N.B: Care must be taken when handling solutions containing high concentrations of guanidine salts due to its chaotropic nature. As with other procedures involving RNA, gloves should be worn at all times to avoid contamination of samples

Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA

(5 ), inflammatory disease (1 ) and carcinogenesis (1 –3 ,6 ). The 8-hydroxy-2-deoxyguanosine lesion (8-OHdG) is often used in the assessment of oxidative DNA damage and has become the de facto marker for oxidative damage to DNA. 8-OHdG