4-Fluoro-2(3H)-benzothiazoleth

Preparation of [3H]Phosphoinositol Standards and Conversion of [3H]Phosphoinositides to [3H]Phosphoinositols

Any chromatographic procedure for the separation of phosphoinositols will require a supply of standards, both for validation of the technique and identification of the products. Tritium-radiolabeled standards are commercially available in 1–

Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold

such as immunocytochemistry. Fluoro-Gold (Fluorochrome Inc., Denver CO) is one such highly flexible fluorescent retrograde marker commonly used for neuronal labeling and neuroanatomical tracing.

Proliferation Assay: [3H] Thymidine incorporation

cells for 2-4 hours then treat. 5) Six-twenty four hours before cells are to be harvested, add 1 µCi/ml [3H]-TdR to each well. Harvesting: 6) Wash cells 2 times with ice cold PBS. 7) Wash cells 2 times with 5% TCA.