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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Rat IgG2a, κ
- 保存条件:
可在 2 至 8 摄氏度下保存 12 个月。请避免长时间暴露在光线下,切勿冷冻。
- 克隆性:
Monoclonal
- 标记物:
Elab Bright Violet 421
- 适应物种:
Mouse
- 保质期:
12个月
- 库存:
999
- 宿主:
Rat
- 应用范围:
FCM
- 浓度:
5 μL/Test
- 规格:
50Tests

Elab Bright Violet 421 Anti-Mouse CD8a Antibody[53-6.7]
| Alternate Names |
T8, Lyt2, Ly-2
|
| Cellular Localization |
Membrane
|
| Clone No | |
| Isotype |
Rat IgG2a, κ
|
| Leadtime |
咨询
|
| Background |
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
|
| Abbre |
CD8a
|
| Swissprot |
P01731
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Clonality |
Monoclonal
|
| Isotype Control | |
| Applications | |
| Form |
Liquid
|
| Concentration |
5 μL/Test
|
| Conjugation |
Elab Bright Violet 421
|
| Conjugation Information |
Elab Bright Violet 421 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 421 nm (e.g., a 450/50 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration Date |
12个月
|
| Shipping |
冰袋
|
| Gene ID |
12525
|

Elab Bright Violet 421 Anti-Mouse CD8a Antibody[53-6.7]Elabscience®上线三种新荧光标记(Elab Bright™ Violet 421、Elab Bright™ Violet 510、Elab Bright™ Violet 650)流式抗体53支。目前,Elabscience®已拥有21种标记的流式抗体。

Elab Bright Violet 421 Anti-Mouse CD8a Antibody[53-6.7]2-8℃保存12个月
Elab Bright Violet 421 Anti-Mouse CD8a Antibody[53-6.7]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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悬液的表型鉴定和功能分析。巨噬细胞没有类似于T细胞(CD4/CD8)那样明确的单一区分抗原,因此需要使用一组标志物的组合来鉴定巨噬细胞。对于小鼠巨噬细胞,常用的组合包括F4/80、CD11b、Ly6C和THY-1等。通过多色荧光标记和流式细胞仪分析,可以在异质性细胞群中精确地鉴定和分选出巨噬细胞。 图5. C57BL/6J腹腔炎症小鼠取腹水样本,使用PE Anti-Mouse/Human/Monkey CD11b Antibody[M1/70](E-AB-F1081D)、Elab Fluor®
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检测。 M2极化:弃去旧培养基,换为无血清培养基饥饿处理16-20 h;再更换为新鲜全培养基静息培养24 h;最后加入IL-4和IL-13(50-100 ng/mL)诱导培养24-48 h,完成M2极化。 流式检测: (1)收集细胞悬液,150 × g离心3 min; (2)弃上清,加入Cell Staining Buffer或PBS缓冲液重悬细胞沉淀,调整细胞密度为1-10×106/mL; (3)取100 μL细胞悬液,每组加入2 μL Purified Anti-Mouse CD
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