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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
联科生物
- 检测方法:
双抗夹心法
- 应用:
ELISA
- 适应物种:
人
- 规格:
48T
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
with the reagent. As a positive control, use cells set aside at the last step of the reprogramming procedure. b. Carry out a reverse transcription reaction using 1 µg of RNA (from step 1, above) and the SuperScript® VILO™ cDNA Synthesis Kit following
#1). Figure 2. Silencer ™ siRNA Validation Data Generated Using Applied Biosystems TaqMan® Gene Expression Assays. The indicated Silencer Validated siRNAs were transfected into HeLa Cells at 30 nM. RNA was isolated 48 hours later and analyzed by one-step
in mammalian cells. Proc. Natl. Acad. Sci. USA 99(9) : 6047-6052. 7. Paul, C.P., Good, P.D., Winer, I., and Engelke, D.R. (2002) Effective expression of small interfering RNA in human cells. Nature Biotechnology 20 : 505-508. 8. Brummelkamp, T.R., Bernards, R
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