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- ITI Biochem
- 美国
- 1245563-12-5
- 2025年07月17日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
4-Bromo-1-chloro-2-[2-(N,N-dimethylamino)ethoxy]benzene
- 库存：
98
- 供应商：
北京孚博
- CAS号：
1245563-12-5
- 规格：
1g
CAS：1245563-12-5
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文献和实验Oligodeoxyribonucleotides Synthesis: Phosphoramidite Approach
of the “phosphorodichloridite” coupling procedure reported earlier by Letsinger and coworkers ( 2 ). Essentially, the approach involved the reaction of the protected deoxyribonucleosides 1a–d (Fig. 1 ) with chloro-( N,N -dimethylamino) methoxyphosphine ( 2 ) in the presence
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
Beta-Gal staining in primary neurons
2 Part b. X-gal (5-bromo-4-chloro-3-indolyl-beta-gal) 40 mg/ml in DMSO Heat part a & b to 37 C Add part b to part a to final 1 mg/ml X-gal. Add part b slowly while mixing, or crystals will form. Filter to remove crystals.
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