- 询价
- ITI Biochem
- 美国
- 213462-02-3
- 2025年07月17日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
4-(4-NITROPHENYL)-4,5,6,7-TETRAHYDROTHIENO[3,2-C]PYRIDINE
- 库存：
98
- 供应商：
北京孚博
- CAS号：
213462-02-3
- 规格：
1g
CAS：213462-02-3
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文献和实验)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C
Two dimensional peptide mapping
will probably go down. After exposure, cut out the membrane piece of interest and soak it immediately in 0.5% PVP-360 (Polyvinylpyrrolidone, MW360) in 100mM acetic acid for 30 min. at 37°C. If you don't do this, your yield will be poor. This may result
. Cells were harvested at 90% confluency and washed to remove contaminants. Cell pellets were lysed for 1 hour at 4°C in buffer containing protease inhibitors and the crude lysates clarified by centrifugation and stored on ice. Confirmation
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