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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
3-Chloro-5-(chlorocarbonyl)phenylboronic acid, anhydride
- 库存:
98
- 供应商:
北京孚博
- CAS号:
957120-24-0
- 规格:
1g
CAS:957120-24-0
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
siliconized slide place two drops of 45% acetic acid and a clean siliconized 18 mm square coverslip. On the coverslip place a small drop (2-3 mm) of fixative( 1/2/3 mixture of lactic acid/water/glacial acetic acid) 3) In the first drop of 45% acetic
Blocking cDNA arrays printed on amine slides
to the 1-methyl-2-pyrolidione. Stir on stir plate to dissolve (1-3 minutes). ____8. Add 10 mls 1M boric acid to the stirring solution once succinic anhydride dissolves. (note: the solution may cloud after adding the boric acid-Brown lab says
In Situ Hybridization Protocols
temperature for five minutes. Fix in 4% paraformaldehyde in DEPC-PBS for 15 minutes. Rinse once in DEPC-water. Place slides in an RNAse-free glass trough with a stir bar. Add 250ml 0.1M RNAse-free triethanolamine-HCl pH 8.0. Add 0.625ml acetic anhydride
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