1,3-Di-tert-butylimidazolium t

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

in a yield of 40% after P (III) oxidation and purification. Complete removal of the nucleobases, phosphates, and 2??O ?tert ?butyldimethylsilyl protecting groups leads to the desired propargylated c?di?GMP diester. Cycloaddition of a biotinylated azide

Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides with Probes Bearing Hydrazine or Amino Groups. II. LPS Activation with a

-4-dimethylaminopyridinium tetrafluoroborate (CDAP). The method yields conjugates with good labeling ratios, preserving the endotoxic activity of the lipid A moiety. Conjugation of smooth-form LPS from Salmonella enterica sv. Minnesota with dansyl hydrazine and horseradish

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

2 Cl 2 ), ACS grade Imidazole, ≥99.5% pure Tert ‐butyldimethylsilyl chloride (TBS‐Cl), >95% pure