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文献和实验Preparation of Affinity Column
Preparation of Affinity Column SEK 5/3/95 1.Add 222.2μl 1M MOPS,pH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final concentration). 2.Read OD205,OD280 (using 0.1M MOPS buffer as blank) 3.Affigel-10 is stored frozen at 4.Filter 1-2
On-Column Ligand/Receptor Derivatization Coupled to Affinity Capillary Electrophoresis
The coupling of on-column derivatization of small molecules to affinity capillary electrophoresis (ACE) has only been realized during the past 5 yr. In this technique, multiple zones of reagent(s) and ligand or receptor are injected
Affinity Column Preparation 亲和层析柱制备
and rotate in the 15 ml tube for at least 5 hours at room temperature.7.Wash away lysine by 4 washes of 10 ml each of PBS.8.Add PBS to the beads and pour them into a small plastic column.Run PBS through the column to pack the beads,and store the column
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