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文献和实验10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
-----------------------Gels:10.5 ml (20%/0.33%)acrylamide/bis acrylamide3.5 ml 10X TGOE buffer1.75 ml 50% glycerol35 microliters 0.5 M DTT20.9 ml H2O0.3 ml 10% ammonium persulfate30 microliters TEMED10X TG0E 500 ml0.25 M Tris 15.1 g Tris1.9 M glycine 71.3 g glycinepH
Measurement of Plasma Renin Activity
Measurement of angiotensin 1, released in plasma by the action of endogenous enzyme renin on endogenous substrate, angiotensinogen is described. Details are given for the generation of angiotensin 1 from plasma using controlled conditions
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