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- 文献和实验
- 技术资料
- 保存条件：
咨询
- 保质期：
咨询
- 英文名：
kb-NB77-78
- 库存：
咨询
- 供应商：
上海甄准生物科技有限公司
- CAS号：
1350622-33-1
- 规格：
2mg
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文献和实验. It had been noted that only λ DNA 37–52 kb in length (or 78–102% of the wild-type length) were efficiently packaged and passaged in vivo. Thus vectors from which the intergenic region had been removed could be positively selected for by the insertion of target DNA
Preparation of total cellular RNA--提取总RNA方法
, 18s @ 1.9kb, 5s @ 160bp)5x Lysis [Final] 2xPK buffer14ml 5M NaCl 140mM 20ml 1M Tris pH7.5 (10mM)750μl 1M MgCl2 1.5mM 5ml 0.5M EDTA5ml 1M Tris pH 8.6 10mM 6ml 5M NaCl2.5ml NP40 0.5% 10ml 20% SDS78ml H2O 59ml H2O
Point Mutation Detection in the Dystrophin Gene
consists of 79 exons, spliced together in a 14-kb mature RNA (1 ,2 ). The protein coding region spans 11,058 bp, and encodes a 3685 amino acid protein with a mol wt of 427 kDa (2 ). Transcripts have been identified in many nonmuscular tissues, initiated
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