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- 文献和实验
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- 保存条件:
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- 英文名:
Biotin LC hydrazide
- 库存:
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- 供应商:
上海甄准生物科技有限公司
- CAS号:
109276-34-8
- 规格:
5mg
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文献和实验Protein Carbonyl Determination Using Biotin Hydrazide
Protein oxidation is a recognized component of aging and a consequence of severe or prolonged exposure to reactive oxygen species (ROS). Direct attack of protein by ROS causes formation of protein-bound carbonyl groups (1 ). These carbonyl
by partial digestion of HA with ovine testicular hyaluronidase, and the oligosaccharides were labeled with biotin hydrazide and purified by a combination of aggrecan G1 domain and avidin affinity chromatography. Hyaladherins were prominently visualized
平衡的Sephadex G25 PD-10预装柱,使之与过碘酸钠分开。收集蛋白峰。(4)向抗体管中加入Biotin-LC-hydrazide(Pierce)至终浓度5mMol/L,置混摇器上室温孵育1h。(5)用含0.02%NaN3的PBS平衡Sephadex G25预装柱,将生物素标记的抗体分子过柱与游离的生物素分开。收集蛋白峰,保存-20℃。 2. 生物素标记DNA片段的制备 作为将与抗体偶联的报告DNA片段,应确保在待测抗原来源的机体DNA中无同源序列,如可选用大肠杆菌的序列作为报告
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