3-chloro-N-[(4E)-3-(3-chloro-4
-methylphenyl)-1-(cyanomethyl)-2-oxoimidazolidin-4-ylidene]-1-benzothiophene-2-carboxamide- 询价
- zzstandard
- 欧美
- ZC-44097
- 2025年07月12日
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- 英文名：
3-(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)-4-[(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)amino]cyclobut-3-ene-1,2-dione
- 库存：
1
- 供应商：
上海甄准
- 规格：
20mg
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
DETECTION OF ß-GALACTOSIDASE The E. coli lacZ gene encoding ß-galactosidase (ß-gal) is the classical
Purification of GST fusion proteins in E.coli GST融合蛋白纯化（方法四）筛选表达
may be stored at 4℃ for up to 1 month.Bulk purification of protein expressed in E.coliPick several colonies of E.coli transformed with the pGEX recombinants into separate tubes containing 2 ml of 2 x YTA medium.Grow o/n at 20~37℃.Add 2 ml culture into 100 ml 2 x
using the GST substrates CDNB (1-chloro-2,4-dinitrobenzene).Centrifuge in a microcentrifuge for 5 min to remove insoluble materials.Save a10 μl aliquots of the insoluble material for analysis by SDS-PAGE.Transfer the supernatants to fresh tubes,Add 20 μl
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