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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken SRC
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken SRC ELISA KIT
ELI-41030c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken SRC ELISA KIT is for the quantitative in vitro determination of Chicken SRC concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken SRC ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken SRC in the sample, this Chicken SRC ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken SRC concentration. The concentration of Chicken SRC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验[3] 将细胞裂解物分离成 RIPA 可溶性和 RIPA 不可溶性组分,发现在两个组分中都可以检测到 TDP-43 的磷酸化片段。同样的,用 RIPA 缓冲液裂解小鼠 MEC 细胞时,磷酸化的 EGFR(pY-1068 EGFR)只在 RIPA 可溶性组分中检测出。然而,磷酸化的 c-Src(pY-416 c-Src)则在 RIPA 可溶性和 RIPA 不可溶组分中都能检测出, 甚至 WT 样品在 RIPA 不可溶组分中的信号比 RIPA 可溶性组分中更强(下图),结果表明如果仅使用 RIPA 可溶性组分
说明书为准。 保存条件及有效期 纤维素 fitc流式ZNF286IHC、FACS、WB、E 1.试剂盒保存:;2-8℃。 2.有效期:6 个月 人一氧化氮(NO)酶联试剂盒说明书 2-甲氧乙基酯 fitc流式ZNF537IHC、FACS、WB、ELISA Rabbit polyclonal to ZNF537 antibody 乙二胺 fitc流式ZNF5IHC、FACS、WB、ELISA Rabbit
多篇 SCI 发现用 RIPA 提蛋白存在问题,我们该如何应对?
90, c-Src, 和 tubulin,表明这些蛋白在 RIPA 不可溶组分中大量存在(下图)。Wang 等 [6] 对比 SDS 加热法和 RIPA 裂解液法从斑马鱼肝脏肿瘤中提取蛋白,发现 RIPA 裂解液提取这些样品时对于大分子量蛋白的提取效率十分低(下图)。Li[7] 对比了从小鼠脾脏和肝脏中提取总蛋白 RIPA 可溶和不可溶性组分以及基于离心管柱法商业试剂盒提取的蛋白质图谱,发现 RIPA 不可溶组分蛋白质图谱与可溶性组分图谱相似,但是不完全相同。这些丢失的不可溶组分覆盖了整个蛋白质图谱分子
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