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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken STAT3
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken STAT3 ELISA KIT
ELI-05653c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken STAT3 ELISA KIT is for the quantitative in vitro determination of STAT3 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken STAT3 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of STAT3 in the sample, this Chicken STAT3 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus STAT3 concentration. The concentration of STAT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验leexuanhao 诸位好,小弟在做药物对癌细胞株的影响,病探讨机制,预实验结果显示,药物对细胞株的增殖是有抑制作用的,进一步探讨抑制机制,选取STAT3,应该ELISA法检测磷酸化水平,之前看过很多文献,知道STAT3是个癌基因,预期结果应该降低P-STAT3的水平,但是结果显示其水平增高,不知道如何解释啊。本人不是分子生物学专业的,平时只能是在临床工作中抽时间看看文献,但现在真是想不明白,请专业的大侠予以指点!万谢! 亦可回复邮件!
,然后将细胞在含有1%BSA(PBA)的PBS中洗4次。用含有PBA溶解的0.1%Triton® X-100的10%驴血清封闭细胞45分钟。然后加入10 μg/mL的一抗,孵育3个小时,之后把细胞在PBA中清洗三次,每次洗5分钟。然后,用5 μg/mL的NothernLightsTM557连接的二抗(R&D Systems)孵育细胞1个小时,然后如上清洗细胞。 STAT3活化水平:用Active STAT3 DuoSet IC®试剂盒(R&D Systems)检测STAT3的活化
说明书为准。 保存条件及有效期 纤维素 fitc流式ZNF286IHC、FACS、WB、E 1.试剂盒保存:;2-8℃。 2.有效期:6 个月 人一氧化氮(NO)酶联试剂盒说明书 2-甲氧乙基酯 fitc流式ZNF537IHC、FACS、WB、ELISA Rabbit polyclonal to ZNF537 antibody 乙二胺 fitc流式ZNF5IHC、FACS、WB、ELISA Rabbit
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