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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Ubiquitin-fold modifier 1,UFM1
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Ubiquitin- fold modifier 1, UFM1 ELISA KIT
ELI-51199c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Ubiquitin- fold modifier 1, UFM1 ELISA KIT is for the quantitative in vitro determination of Chicken UFM1 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Ubiquitin- fold modifier 1, UFM1 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken UFM1 in the sample, this Chicken Ubiquitin- fold modifier 1, UFM1 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken UFM1 concentration. The concentration of Chicken UFM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Studies of the Ubiquitin Proteasome System
both in vitro and in cells. Because another protein modifier, NEDD8, itself regulates aspects of the ubiquitin system, basic protocols on neddylation are also included in this unit. Keywords: Ubiquitin; ubiquitylation; proteasomal degradation; protein
PCR PRIMER DESIGN AND REACTION OPTIMISATION
opportunities are greater, and background noise increases; also, increased degeneracy means concentration of the individual primers decreases; thus, greater than 512-fold degeneracy should be avoided. However, I have used primers with as high as 256- and 1024
通用的HaloTag™反应联结子,结合HaloTag™蛋白;(2)一个功能基团,例如荧光染料或亲和媒介。 能够共价和特异性结合多种合成的报告基团和亲和配基的特性,使得HaloTag™蛋白能够用于检测和亲和结合或固相化固定目的蛋白。 SUMO SUMO标签蛋白是一种小分子泛素样修饰蛋白(Small ubiquitin-like modifier),是泛素(ubiquitin)类多肽链超家族的重要成员之一。在一级结构上,SUMO与泛素只有18%的同源性,然而两者的三级结构及其生物
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