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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken 60S ribosomal protein L27,RPL27
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken 60S ribosomal protein L27, RPL27 ELISA KIT
ELI-41361c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken 60S ribosomal protein L27, RPL27 ELISA KIT is for the quantitative in vitro determination of Chicken RPL27 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken 60S ribosomal protein L27, RPL27 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken RPL27 in the sample, this Chicken 60S ribosomal protein L27, RPL27 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken RPL27 concentration. The concentration of Chicken RPL27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验with 250 µl Pierce Coomassie protein assay reagent in 96-well ELISA dishes and reading at 650 nm on a plate reader).The foregoing affinity purification steps were carried out at 4ºC.The proteins were dialyzed against PBS and stored at –20ºC.Protein
, where the morphology of the germ line and the progeny produced are of lower quality. The use of egg plates is not recommended, because it is rather difficult to reduce the high protein and lipid background, which is a result of contaminating chicken yolk.
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
6060 NM_000988 Homo sapiens ribosomal protein L27 (RPL27), mRNA 6101 NM_001004 Homo sapiens ribosomal protein, large P2 (RPLP2), mRNA 5924 NM_001003 Homo sapiens ribosomal protein, large, P1 (RPLP1), mRNA 10300 NM_000405 Homo sapiens GM
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