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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken UBE2V2
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken UBE2V2 ELISA KIT
ELI-17736c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken UBE2V2 ELISA KIT is for the quantitative in vitro determination of Chicken UBE2V2 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken UBE2V2 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken UBE2V2 in the sample, this Chicken UBE2V2 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken UBE2V2 concentration. The concentration of Chicken UBE2V2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验说明书为准。 保存条件及有效期 纤维素 fitc流式ZNF286IHC、FACS、WB、E 1.试剂盒保存:;2-8℃。 2.有效期:6 个月 人一氧化氮(NO)酶联试剂盒说明书 2-甲氧乙基酯 fitc流式ZNF537IHC、FACS、WB、ELISA Rabbit polyclonal to ZNF537 antibody 乙二胺 fitc流式ZNF5IHC、FACS、WB、ELISA Rabbit
共沉淀方法分离特定蛋白质及其结合蛋白(包括被泛素化修饰的蛋白)。分离出的蛋白经SDS电泳后用泛素抗体进行western blot分析,可以鉴定具体哪个蛋白发生了泛素化修饰以初步判断某蛋白具有泛素化功能。 2体外泛素标记突变位点 将研究蛋白(被泛素化的蛋白)通过蛋白提取试剂盒提取内源性蛋白或通过基因工程技术重组表达后提纯。用k63或k48连接的泛素链肽段系列酶E1、E2、E3在适当条件下体外孵育,然后用western blot和泛素化抗体检测。该方法可以明确
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
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