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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Centrosomal protein of 63 kDa,CEP63
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Centrosomal protein of 63 kDa, CEP63 ELISA KIT
ELI-24860c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Centrosomal protein of 63 kDa, CEP63 ELISA KIT is for the quantitative in vitro determination of Chicken CEP63 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Centrosomal protein of 63 kDa, CEP63 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken CEP63 in the sample, this Chicken Centrosomal protein of 63 kDa, CEP63 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken CEP63 concentration. The concentration of Chicken CEP63 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验基于膜的检测试剂研发人员应该严格考虑影响蛋白质在硝酸纤维素膜上结合众多因素,其中包括原材料的固有属性和处理过程等。随着胶体金标记技术的发展、研发人员对胶体金快速检测技术深入研究,市场上基于膜的快速免疫层析检测产品的种类迅速增多。虽然免疫层析产品的包装设计种类繁多,实际上目前主流的商品化检测试剂盒主要下面两种形式。最常用的检测形式为侧向层析或者层析定量,这种检测形式通常应用于诊所或者由直销客户检测。另一种检测形式为竖向斑点渗滤,该检测形式需要较强的操作技巧,仅限于研究使用。不管试剂采用那种检测
, the amount of enzyme should be increased. Problem: What can be done to improve ChIP when using chicken antisera that do not bind well to either protein A or protein G? [Step 47] Solution: We recommend adding 5 µg of a rabbit anti-chicken antiserum
/bbs/post/view?bid=65&id=4022205&sty=1&tpg=63&age=0 17[求助]各位高手,有没有谁做过合成肽做为ELISA包被抗原的? http://www.dxy.cn/bbs/post/view?bid=65&id=4022016&sty=1&tpg=63&age=0 18 请教噬菌体外壳蛋白的分离技术 http://www.dxy.cn/bbs/post/view?bid=65&id=4020115&sty=1&tpg=63&age=0 19[求助
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