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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Histone H2A deubiquitinase MYSM1,MYSM1
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Histone H2A deubiquitinase MYSM1, MYSM1 ELISA KIT
ELI-20017c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Histone H2A deubiquitinase MYSM1, MYSM1 ELISA KIT is for the quantitative in vitro determination of Chicken MYSM1 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Histone H2A deubiquitinase MYSM1, MYSM1 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken MYSM1 in the sample, this Chicken Histone H2A deubiquitinase MYSM1, MYSM1 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken MYSM1 concentration. The concentration of Chicken MYSM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination
of such modifications is histone ubiquitination, which occurs predominately on H2A and H2B. Recent studies have highlighted important regulatory roles of H2A ubiquitination in Polycomb group proteins-mediated gene silencing (Wang et al., Nature 431:873–878, 2004; Joo et
,为此CST公司推出了可用于蛋白功能检测的乙酰化组蛋白系列抗体,圆满的解决这个问题。因为乙酰化与蛋白的活性相关,因此通过乙酰化的系列抗体特异性地识别靶蛋白的乙酰化形式,可以反映出靶蛋白质的活性水平,达到研究蛋白功能和调节的目的。而其他的抗体仅能反映出该蛋白质的表达量或表达水平,不能反映出蛋白的活性状态。乙酰化系列抗体:Catalog#Product DescriptionSourceApplications2576Acetyl-Histone H2A (Lys5) AntibodyRabbitW IP
的DNA绕由H2A, H2B, H3和H4各两分子形成的八聚体约两圈的粒状结构, 其中H3和H4各两分子形成的四聚体位于八聚体的中间, 其两端均与H2A和H2B各一分子形成的二聚体结合。第五种组蛋白H1或称H5与连接两核小体之间的DNA结合, 并与相邻两核小体首尾相联, 以稳定核心组蛋白与DNA双链的结合。Crane等(1997)对H1与核小体的结合位置提出新的看法〔2〕, 认为染色质的高级结构是以核小体为基本结构单位的一级结构经过进一步的盘绕、折叠而形成。由于染色质是真核细胞遗传信息的载体, 真核
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